Part:BBa_K3165003

PcyA (for E. coli)

PcyA or Phycocyanobilin ferredoxin oxidoreductase is the enzyme responsible for carrying out the second step in the biosynthesis of Phycocyanobilins (PCB). PcyA catalyzes the conversion of biliverdin to phycocyanobilin, the immediate precursor of cyanobacterial phytochromes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

The enzyme phycocyanobilin ferredoxin oxidoreductase catalyzes the four-electron reduction of biliverdin IX alpha (produced by the enzyme heme oxygenase 1: (BBa_K3165002) ) to phycocyanobilin (2-electron reduction at both the A and D rings), the immediate precursor of cyanobacterial phytochromes. The reaction proceeds via an isolatable two-electron intermediate: 181,182-dihydro biliverdin.

Usage

This part can be used in association with heme oxygenase (BBa_K3165002) to couple the two stages of phycocyanobilin biosynthesis. The phycocyanobilin biosynthesis unit (comprising of ho1 and pcyA) can be used along with the CcaSR system to generate a functional optically regulated gene expression system.

References :

(1) https://www.uniprot.org/uniprot/Q55891
(2) Okada, K. (2009). HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis. FEBS Letters, 583(8), 1251–1256. DOI:10.1016/j.febslet.2009.03.052